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文献和实验4.1 ), using 15% acrylamide (15%T:5%C) in 2.5 M urea and 0.9 M acetic acid. Set up the gel in the electrophoresis unit and run the gel at 2 mA/gel for 2 hours with no sample, using 0.9 M acetic acid for the running buffer. Dissolve
Determining the Direction of Replication Fork Movement
to completion--see The NEB Transcript, Vol. 3 No. 1, March, 1991, pg 8-9.) 1. After staining and photographing the first dimension gel, excise the lane and place it in a disposable pipette reagent reservoir from Costar (#4870; Cambridge MA). These plastic
Generation and Identification of Arabidopsis EMS Mutants
(MA) linked to the mpi locus or mutant phenotype, more markers near MA are tested to identify recombinants. The recombinants indicate the interval in which the mpi is located. Additional recombinants and molecular markers are then required to narrow
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