- ￥2277
- 赛默飞（Thermo Fisher）
- MA1036X
- 2025年07月15日
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MONOCLONAL ANTIBODY- (14C8)
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文献和实验----------------------- Screening for Protein-Protein Interactions. Protein G was from Pierce (Rockford, IL) and BODIPY-FL-Goat-anti-Mouse IgG was from Molecular Probes (Eugene, OR). IKBalpha and p50 were kindly provided by T. Maniatis (Harvard University, Cambridge, MA
Immunostaining of Paraffin Sections
), and then to PBS (2 x 5 min). Insert sections in Coplin jars containing ‘TUF’ (Target Unmasking Fluid; Signet Labs [Dedham MA] #1050) preheated to 80-90°C. Incubate in TUF for 10 min at 80-90°C. Let jar cool 5 - 10 min, then rinse two times in ddH
luminol and oxidizing reagents (Pierce or NEN, Boston, MA) for 5 min at RT. Develop by X-OMAT autoradiography per recommendation of the manufacturer.
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