- ￥5963
- 赛默飞（Thermo Fisher）
- 339194
- 2025年07月13日
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- 库存：
100
- 规格：
100 µg
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文献和实验T-Cell Activation Using mAb to CD3
can be performed. For costimulation studies using antibodies to other antigens, a suboptimal activation with anti-CD3 may be required. To achieve suboptimal activation via anti-CD3, a 0.5-0.1µg/ml 145-2C11 antibody solution can be used. Dispense 50µl
Dissolve Proteinase K (Sigma Catalog No. P-4914) in dH2O for a concentration of 20mg/ml. 1% BSA/PBS 1g Fraction V BSA in 100ml 1x PBS. Mix in a beaker with low heat. Aliquot in 15ml tubes and store at -20°C. Hematoxylin 75ml Gill's
150µl of working dilution. Incubate sections in a humid chamber for 1 hour, RT. Blot off antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of the secondary antibody (Horse anti-mouse IgG-Vector Catalog No. BA2001) in 1.0%
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