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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Assessment of the Recycling of the Membrane-Bound Chemokine, CX3CL1
of mammalian cells with plasmids encoding the expression of green fluorescent protein-tagged CX3 CL1; (2) immunofluorescence antibody labeling as well as fluorescence recovery after photobleaching to study internalization of CX3 CL1 by endocytosis; and (3) acid
5. Electorophoresis with 100-130 V in 0.25X TBE buffer for 3 hours. You may use 18 cm-height gel system and stop running after dye front comes to 12 cm point from the top. When a gel was run with 130 V, the current was less than 20 mA. You could run a gel
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