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文献和实验Assessment of the Recycling of the Membrane-Bound Chemokine, CX3CL1
of mammalian cells with plasmids encoding the expression of green fluorescent protein-tagged CX3 CL1; (2) immunofluorescence antibody labeling as well as fluorescence recovery after photobleaching to study internalization of CX3 CL1 by endocytosis; and (3) acid
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
Western Analysis of Transgenic Plants
are transferred to a solid support, which is normally nitrocellulose or polyvinylidene fluoride (PVDF [Millipore, Bedford, MA]). Immunological detection of proteins transferred to a solid support combines ease of handling with accessibility of antibodies
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