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文献和实验. Add 50ul of each of the above dilutions to 25ul of 3x strength titre of antibody (i.e. a solution 3 x the titre giving 50% inhibition on direct ELISA), in sealed tubes. 3. Incubate overnight at room temperature. 4. Set up competition
DAY 1 1. Coat Nunc immuno-module plates overnight, in usual manner. 2. Set up competition assay between antibody and competing substance :- Prepare a 1:2 serial dilution of the competitive substance from 480 to 0.4 ng/ml. Add 50ul
Generation of Recombinant Multimeric Antibody Fragments for Tumor Diagnosis and Therapy
Single chain recombinant antibody fragments can multimerise to provide high binding avidity and unique specificity for target antigens and can be used to replace the parent antibody or Fab derivatives (1 ). A unique advantage
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