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文献和实验Inducible microRNA-Mediated Knockdown of the Endogenous Human Lamin A/C Gene
inactivation both in vitro and in vivo. Here, the generation of a conditional RNAi cell line for microRNA (miRNA)-mediated downregulation of the endogenous lamin A/C gene is described. A tet-responsive transcription unit, encoding a designed miRNA against human
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
., Graves, D.J., DeMaggio, A.J., Hoekstra, M.F., Blenis, J., Hunter, T., and Cantley, L.C. 1996. A structural basis for substrate specificities of protein Ser/Thr kinases: Primary sequence preference of. casein kinases I and II, NIMA, phosphorylase kinase
Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage
Phospho-specific antibodies have become very useful reagents for study of signal transduction pathways. This chapter describes the production of phospho-specific antibodies and their use to assess individual phosphorylation events in vivo
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