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文献和实验Measurement of TNF and iNOS mRNA Using cDNA-Equalized Reverse Transcriptase PCR
Since the polymerase chain reaction (PCR) for DNA amplification was first introduced in 1985 (1 ), the combination of reverse transcription with subsequent PCR amplification of the cDNA (RT-PCR) has been an increasingly utilized technique
Measurement of eNOS and iNOS mRNA Expression Using Reverse Transcription Polymerase Chain Reaction
)-dependent endothelial NOS (eNOS) and neuronal NOS (nNOS) and an inducible Ca2+ /CaM-independent NOS (iNOS). If NO is produced in insufficient amounts, or if it reacts with other compounds, it often escapes detection. Because the failure to detect
lishuning2010 我的实验中,Western blot显示蛋白水平随时间延长表达增加,但是RT-PCR结果却显示随时间表达减少,如何解释? zhujoker 那看一下是不是蛋白降解减少? bigbang_0_0 有可能蛋白质的稳定性增加或者翻译效率增加,增加幅度大于mRNA降低所带来的蛋白理论水平降低的幅度 lishuning2010 非常
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