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- 文献和实验
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- 库存:
100
- 规格:
100 µg
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文献和实验the column with 3 l of Buffer D using a fast flow rate. 2. Elute the column with a 4 l gradient from 100 mM KCl to 500 mM KCl in Buffer D. Collect 25 ml fractions (400 drops/fraction). Actin-containing fractions are identified by dot blot using mAb C4
LABELING MUSCLE ACTIN WITH IATR
. 4.Collect actin from dialysis tubing,add 100 mM KCl and 2 mM MgCl2 to induce polymerization.Let sit on ice for 20 min. 5.Get >=1 mg IATR in a test tube.Add 100-200 µl acetone and make a fine slurry by grinding particles against the tube with a pipet. 6.Add
Labeling Muscle Actin with 5-iodoacetamidofluorescein
1 overnight. 4.Collect actin from dialysis tubing,add 100 mM KCl and 2 mM MgCl2 to induce polymerization.Let sit on ice for 20 min. 5.Get 2.4 mg IAF in a test tube.Add 100-200 µl acetone and make a fine slurry by grinding particles against the tube
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