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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 规格:
100 µg
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文献和实验过程中,一抗应在封闭缓冲液 (TBS/0.3%Triton. X–100/5% NGS) 中稀释。抗体:Phospho–Akt (Ser473) (D9E) XP. Rabbit mAb #4060、Phospho–EGFReceptor (Tyr1173) (53A5) Rabbit mAb #4407样本:石蜡包埋人类乳腺肿瘤(上图)和 HCC827 异种移植物(下图)抗体稀释:SignalStain. 抗体稀释液(左)或 TBST/5%NGS(右)检测—检测系统传统的 IHC 检测
.N. Cabili, C. Trapnell, L. Goff, M. Koziol, B. Tazon-Vega, A. Regev, J.L. Rinn, Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses, Genes Dev 25(18) (2011) 1915-27. [2] G.D. Yang, X.Z. Lu
Single-molecule analysis of DNA replication by molecular combing
of 400 µg/ml (or 40 µg/ml if cells express hENT1), at least 15 minutes before releasing cells into S phase (see note 1). Add HU to a final concentration of 200 mM (15 mg/ml) and release cells from α-factor with the addition of 50 µg/ml Pronase
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