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100UG
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文献和实验过程中,一抗应在封闭缓冲液 (TBS/0.3%Triton. X–100/5% NGS) 中稀释。抗体:Phospho–Akt (Ser473) (D9E) XP. Rabbit mAb #4060、Phospho–EGFReceptor (Tyr1173) (53A5) Rabbit mAb #4407样本:石蜡包埋人类乳腺肿瘤(上图)和 HCC827 异种移植物(下图)抗体稀释:SignalStain. 抗体稀释液(左)或 TBST/5%NGS(右)检测—检测系统传统的 IHC 检测
Intracellular Immunofluorescent Staining for Flow Cytometry
PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53
785.2 H2 O [ml] 948.35 895 840.65 784.6 727.15 668.2 607.55 545.7 %(w/w) 9.379 17.988 25.940 33.350 40.295 46.846 53.070 58.998 H2 O %(w/w) 90.621 82.012 74.060 66.650 59.705 53.154 46.930 41.002 p (g
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