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文献和实验PriCells: Primary cells culture with supplement of vascular endothelial growth factor (VEGF)
8) splice site - the proximal splice site (denoted VEGFxxx) or distal splice site (VEGFxxxb). In addition, alternate splicing of exon 6 and 7 alters their heparin-binding affinity, and amino acid number (in humans: VEGF121, VEGF121b, VEGF145, VEGF165, VEGF165
【原创/分享】GS115毕赤酵母表达外源蛋白较详细步骤!!!(从验证到阳性克隆的筛选)
公司产品 PCR仪 5. 各种培养基 (1) LB培养基 Peptone 10g 酵母抽提物 5g 氯化钠 10g 溶于蒸馏水,定容到1000ml,高压灭菌,121度20min。如做平板,则加15g 琼脂粉,待冷却至50度左右加入AMP至终浓度为50ug/ml。 (2) YPD培养基 Peptone 2g 酵母抽提物 1g 溶于蒸馏水,定容至96ml,高压灭菌121度20min,待冷却至室温后,加入4ml50%葡萄糖。做平板则加15g琼脂粉。 (3) MD平板 称
In Situ Hybridization Protocols
minutes. Wash twice in DEPC-PBS at room temperature for 5 minutes. Treat slides with 50ug/ml Proteinase K in PK buffer at room temperature for between 8-15 minutes depending on the age of the embryo. Wash once in DEPC-PBS at room
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