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文献和实验2 x2H2 O 1M 147.02g/M 13.54 7.351g 29.40g 44.11g H2 O mQ 86.46 46.92ml 187.7ml 281.5ml Conc. Stock %(w/w) 50ml 200ml 300ml CaCl2 x 6H2 O 1M 219.08g/M 20.18 10.95g 43.82
Intracellular Cytokine Staining Protocol
28 (2ug/ml soluble) 5hrMonensinJES5-16E3IL-12mouse PECmIFNg (100ng/ml) (2hr)/LPS (100ng/ml) (22hr)2hr/22hr-MonensinC17.8GM-CSFmouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble
., and Stern, D. B. (2000) Processing and degradation ofchloroplast mRNA. B io c h im ie 82, 573-582. 3. Barkan, A. and Goldschmidt-Clermont, M. (2000) Participation of nuclear genesin chloroplast gene expression. B io c h im ie 82, 559-572. 4. Sun,
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