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- 赛默飞（Thermo Fisher）
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- 2025年07月14日
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
medium for OP9 cells (for Protocols 1, 2) Leukemia inhibitory factor (LIF; 10 µg/mL; Chemicon LIF2010) (for Protocol 2; see Step 20) Lympholyte-M (Cedarlane CL5120) (for Protocol 3) Mice, 4-8 wk old (for Protocol 4; see Step 69) Mice, fetal
Construction and Characterization of Adenovirus Vectors
), 1.25 g/mL and 1.35 g/mL, in dialysis buffer Prepare each gradient solution separately. Sterilize by filtration through a 0.2-µm filter. Chloroform:isoamyl alcohol (24:1) Citric saline (2X) For 1X citric saline, dilute the 2X
Purification Of Anti Peptide Antibod
Purification Of Anti Peptide Antibody Claire Walczak January 1996 Protocol: All steps up to the dialysis at rt. Pour column in TBS (=0.15M NaCl, 20mM TrisCl pH 7.4). We use a 5 ml column for 25
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