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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
9 cells may lift as a sheet, which will be broken up by forceful pipetting. 45. Filter the cells through a 40-µm cell strainer. 46. Wash the well with 3 mL of PBS, filter the suspension through the same cell strainer
of 5-methylcytosine in DNA by the chloroacetaldehyde reaction [J]. Biotechniques, 1999, 27: 744-6,748-50,752.[25]Frommer M, McDonald L E, Millar D S, et al. A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA
一、体外转录 在无菌1.5 ml 微型离心管中,结合以下内容: 4 ul 5倍转录缓冲液 2 ul 0.1 M DTT 4 ul 2.5 MM NTP(A,C,G) 0.8 ul RNA酶抑制剂(25 U/ul)RNA酶抑制剂(Promega)中 100 UM冷UTP 1 ul/ug UL线性DNA模板 5 ul 10 UCI / UL P32 UTP(800Ci/mmol) 1 ul RNA聚合酶SP6,T7或T3 总体积〜20 ul。
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