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文献和实验Intracellular Cytokine Staining Protocol
: T cytotoxic, CD8+ CD8 53-6.7 14-0081 mature and developing T cells T helper, CD4+ CD4 RM4-5 14-0042
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
9 cells may lift as a sheet, which will be broken up by forceful pipetting. 45. Filter the cells through a 40-µm cell strainer. 46. Wash the well with 3 mL of PBS, filter the suspension through the same cell strainer
In Situ Hybridization Using Digoxigenin Labeled Probes
siliconized slide place two drops of 45% acetic acid and a clean siliconized 18 mm square coverslip. On the coverslip place a small drop (2-3 mm) of fixative( 1/2/3 mixture of lactic acid/water/glacial acetic acid) 3) In the first drop of 45% acetic
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