- ￥3893
- 赛默飞（Thermo Fisher）
- 500-P139BT-50UG
- 2025年07月11日
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- 库存：
100
- 规格：
50 UG
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文献和实验and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC
then recovered and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1% b-mercaptoethanol, 0.02% glusulase and 5ug/ml zymolyase. The suspension
). Immediately scrape the cells off the plate with a rubber policeman. Put the extract into an eppendorf and boil for 5 min., cool on ice. Load onto 10% SDS-PAGE. I usually load about 1/5 of the lysate from a 60
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