MO CD100 BMA12 PUR MAB-100UG-4

Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞

(one bottle) in 250 ml sterile H2O (tf 20ug/ml) store at 4C, re-use for a few months.PC12 culture media DMEM (lab home-made) 10% Horse serum 5% Fetal Bovine Serum (calf serum may also be fine) 2mM glutamine (1/100 from lab stock) penn/strep (1/200

如何使PC12细胞贴壁更牢

PBS洗两遍以彻底去除H2O2及血清的影响，只是这样一来细胞会丢失很多,该如何解决？丁香园midas的观点： 1.增加PLL的浓度，50－100ug/mg 2.没有必要把培养液去掉，并用PBS洗！H2O2及血清对MTT的影响好像 还没有见到相关报道。 丁香园liandchu的问题： 我培养的细胞是pc12细胞,这种细胞不好贴壁,我用多聚赖氨酸包被,但我不知道多大分子量多聚赖氨酸及浓度去包被效果好?, 丁香园luopeng的观点： 用赖氨酸包被培养板和培养瓶时，应该选用多聚左旋赖氨酸

Dual- and Triple-Color Fluorospot

. Spontaneous production is determined by incubating the same number of cells in the absence of antigen. A polyclonal activator, such as anti-CD3 mAb (100 ng/ml) or phytohemagglutinin (PHA; 1–10 μg/ml), should normally be used as a control for cell viability