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文献和实验miRNA microarrays(McManus Lab)(芯片)
://www.ambion.com/techlib/prot/bp_12200.pdf) clean-up kit or by ethanol precipitation (http://www.ambion.com/techlib/misc/acetate_precip.pdf) (Note: Do not use a glycogen carrier). or 2) Purifying small RNA on a denaturing acrylimide gel
RNase Protection Assay(RPA)操作步骤
is a complete system available for detecting and quantifying transcripts. This system includes: Multi-Probe Template Set In Vitro Transcription Kit RPA Kit Individual components may be purchased either separately or together as the RPA
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
) Plasmid miniprep kit (e.g., Wizard Plasmid Purification System, Promega), including resuspension buffer, nuclease removal buffer, wash buffer, elution buffer, and spin columns
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