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100
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100 µg
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文献和实验LABELING MUSCLE ACTIN WITH IATR
. 4.Collect actin from dialysis tubing,add 100 mM KCl and 2 mM MgCl2 to induce polymerization.Let sit on ice for 20 min. 5.Get >=1 mg IATR in a test tube.Add 100-200 µl acetone and make a fine slurry by grinding particles against the tube with a pipet. 6.Add
Labeling Muscle Actin with 5-iodoacetamidofluorescein
1 overnight. 4.Collect actin from dialysis tubing,add 100 mM KCl and 2 mM MgCl2 to induce polymerization.Let sit on ice for 20 min. 5.Get 2.4 mg IAF in a test tube.Add 100-200 µl acetone and make a fine slurry by grinding particles against the tube
for isolation of Erythrocyte Actin 1. Collect blood from animal using 20 ml/100 ml whole blood acid citrate/dextrose (0.085 M sodium citrate, 0.065 M citric acid 2% dextrose) as anti-coagulate. 2. Centrifuge blood at for 10 min at 4300 x g and remove
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