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文献和实验In Situ Hybridization Protocols
minutes. Wash twice in DEPC-PBS at room temperature for 5 minutes. Treat slides with 50ug/ml Proteinase K in PK buffer at room temperature for between 8-15 minutes depending on the age of the embryo. Wash once in DEPC-PBS at room
Anderson Lab In Situ Hybridization Protocols
in 4% paraformaldehyde in DEPC-PBS at room temperature for 20 minutes. Wash twice in DEPC-PBS at room temperature for 5 minutes. Treat slides with 50ug/ml Proteinase K in PK buffer at room temperature for between 8-15 minutes depending on the age
, 0.1mM ßME. plus 3 U/mL Epo, 50 ng/mL Tpo, 100 ng/mL SCF, 100 ng/mL IL-6, ? ng/mL IL-3. Antibodies for Lineage Depletion: (Pharmingen) Antibodies for Sorting: (Pharmingen monoclonal rat anti-mouse)
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