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文献和实验and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC
Rodent Models to Evaluate Anti-obesity Drugs
This article describes the use of rodent models to evaluate the anti-obesity potential of novel drugs. Although drugs can reduce body weight by altering energy input or energy output, we have focused on the discovery of compounds reducing
then recovered and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1% b-mercaptoethanol, 0.02% glusulase and 5ug/ml zymolyase. The suspension
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