- ￥30913
- 赛默飞（Thermo Fisher）
- 500-P84-1MG
- 2025年07月13日
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- 库存：
100
- 规格：
1 MG
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文献和实验Single-molecule analysis of DNA replication by molecular combing
of 400 µg/ml (or 40 µg/ml if cells express hENT1), at least 15 minutes before releasing cells into S phase (see note 1). Add HU to a final concentration of 200 mM (15 mg/ml) and release cells from α-factor with the addition of 50 µg/ml Pronase
Immunohistochemistry (IHC) of Thymus Frozen Sections
Materials Cold Acetone (-20ºC) in Coplin jars PBS, BenchKote paper, plastic lids Anti-digoxigenin-HRP diluent: 1% BSA in PBS (store 10 mL aliquots of BSA at -20ºC) Anti-rat-digoxigenin diluent: 5% Milk powder, 10% mouse serum, 10% goat
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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