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文献和实验and SLE in murine models. Given the broad involvement of deregulated production of IL-17 and IL-21 in autoimmunity, many of these techniques could also be valuable for the investigation of these pathways in murine models of other autoimmune diseases.
RNA Purification Protocol for 0.1-2.5 mg of Animal Tissue
设备 1. Microcentrifuge capable of 13,000 x g 2. Sterile 0.5 mL microcentrifuge tubes 3. Ice bath 4. Microfuge tube pestle 实验步骤 1. Dissect tissue quickly and freeze in liquid nitrogen. Storage at -70°C
in primary murine bone marrow-�derived myeloid cells. The protocol encompasses cell handling, inflammasome activation and inhibition, as well as the detection of IL-1β, caspase-1, and IL-1α by ELISA and Western blot.
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