- ￥7069
- 赛默飞（Thermo Fisher）
- 3630-MSM6-P1ABX
- 2025年07月16日
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- 库存：
100
- 规格：
100 UG
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文献和实验Method for Single-Cell Electroporation
transferred into the cell through these pores. Following pulse termination, pores reseal over 10s to 100s of ms. SCE is a powerful transfection method since it readily allows the delivery of multiple genes each carried by independent plasmids
GENERAL MAINTENANCE INSTRUCTIONS FOR IMAGINAL DISC CELL LINES
The complete M3 medium has the following additives. 2% FBS 2.5% fly extract 5 ug/ml insulin 1/2 x penstrep (100 x bottle = 5000 ug/ml) Adding insulin. Insulin (Sigma, I-6634) Dissolve 5mg insulin in 0.3ml of 0.01N HCl each time
2O 1.75 ml3.5ml1X Lysis Buffer -- 4℃Cool eppys -- 4℃ put 4x # of tubes neededPBS -- 4℃Stimμlation -- 37℃ 10' NGF=100ng/ml à first aspirate 5 ml!KCl=5 ml to 10 mlLyse -- on ICE TRAY Stop stimμlationasp.Media/wash 2XPBS/ +500l lysis bufferscrape
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