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维百奥生物库存现货,特价销售CellTiter-Glo® Luminescent Cell Viability Assay 100ML!
| 产品信息
品名:CellTiter-Glo® Luminescent Cell Viability Assay 100ML
品牌:Promega
货号:G7572
规格:100ml
| 产品描述
The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent and mixing.

The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® Assay generates a "glow-type" luminescent signal, which has a half-life generally greater than five hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch mode processing of multiple plates. The unique homogeneous format avoids errors that may be introduced by other ATP measurement methods that require multiple steps.

The CellTiter-Glo® Luminescent Cell Viability Assay is a popular and trusted method used in a wide range of applications.
| 产品特点
· A homogeneous "add-mix-measure" format reduces the number of steps
· Higher level of detection requires fewer cells per assay
· Process plates consecutively with a very stable luminescent signal
· Integrates with the MyGlo™ Reagent Reader
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文献和实验microscope Protocol prepare cell suspension at a high concentration (ca 106 cells/ml) take a clean hemocytometer slide and fix the coverslip
Primary Neuron Viability Assay
ProtocolsDescription Fluorescence based asssay for establishing neuronal viability in cell culture monolayers. Procedure Monolayer cultures of rat embryonic cortical neurons were prepared and plated at a density of 2-5 x 105 cells
Tetrazolium (MTT) Assay for Cellular Viability and Activity
In the course of examining the effects on cells of polyamines, their metabolites, and polyamine analogs, it is often necessary to make some measure of cellular activity as an indicator of cell damage or cytotoxicity. One of the simplest
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