- ￥3605
- 赛默飞（Thermo Fisher）
- 3514-MSM10-P0
- 2025年07月16日
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文献和实验Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
to the kappa 1a of Y3 (eg. DA which is kappa 1b) to allow later screening of myeloma light chain loss variants (if required). b) The final boost should be by the intravenous route, four days before the fusion, to ensure maximal activity in the spleen.
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