- ￥3528
- 赛默飞（Thermo Fisher）
- 3439-MSM2-P0
- 2025年07月14日
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- 库存：
100
- 规格：
20 UG
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文献和实验Sequenase[TM] catalyzed sequencing with dye-labeled terminators
Single-stranded dye-terminator reactions required approximately 2 ug of phenol extracted M13-based template DNA. The DNA is denatured and the primer annealed by incubating DNA, primer, and buffer at 65degC. After the reaction cooled
Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
and volumes for ppt.need 1.4ml ppt/100mm (half is 2x HeBS, half is DNA/CaCl2 mixture).20-30ug of total DNA required per 100mm plate.5-20ug reporter plasmid.3-4ug alpha-globin internal control.pUC19 to bring to 20-30ug total DNA.Aliquot volume of 2x HeBS
Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌
its "pores" and retain the plasmid/cosmid DNA. Add 900 μl of sterile 1X LBM (or SOC for DH5- ALPHA) to each tube and continue incubating at 37 degrees C for 30 minutes. 5.Because intact plasmids/cosmids transform efficiently (approximately 1x10e7 per ug
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