- ￥6057
- 赛默飞（Thermo Fisher）
- 200-4343-0100
- 2025年07月15日
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- 详细信息
- 文献和实验
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- 库存：
100
- 规格：
100 uG
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文献和实验ELISA (Enzyme-Linked ImmunoadSorbent Assay)
and PSAR (Peroxidase-conjugated Swine Anti Rabbit) for Ab raised in rabbits. 10) Wash 4x with 1x PBS containing 0.01% tween. 11) Add substrate (100ul/well). 12) Incubate for 1-5 mins in the dark at RT. Check reaction for colour change. Wells
(交流)ELISA (Enzyme-Linked ImmunoadSorbent Assay)
with antigen stock solution (1ug/ml in PBS, 50ul/well) for at least 24hrs or over the weekend at 4°C or O/N at 37°C.3) Remove antigen and wash 3x with 1x PBS.4) Block with 1% BSA (100ul/well) in 1x PBS for at least 1hr at 37°C.5) Remove BSA by flicking plate
ELISA (Enzyme-Linked ImmunoadSorbent Assa
is from colourless to blue (yellow when acid is added). 2) Coat plate with antigen stock solution (1ug/ml in PBS, 50ul/well) for at least 24hrs or over the weekend at 4°C or O/N at 37°C. 3) Remove antigen and wash 3x with 1x PBS. 4) Block with 1% BSA (100
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