- ￥99 - 999
- Sigma
- 进口
- 1667202-200MG
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
现货
- 供应商：
联硕生物
- CAS号：
72-19-5
- 规格：
1瓶
一般描述
This product is provided as delivered and specified by the issuing Pharmacopoeia. All information provided in support of this product, including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia.
分析说明
These products are for test and assay use only. They are not meant for administration to humans or animals and cannot be used to diagnose, treat, or cure diseases of any kind.
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文献和实验μL 体系 2× MasterMix 25 μL 上游 Primer （ 10 μM 1-5 μL 下游 Primer （ 10 μM ） 1-5 μL 模板 lamid DNA ～ 50 ng genomic DNA ～ 1000 ng cDNA ～ 500 ng 粗提液 ～ 2 μL 灭菌蒸馏水至 50 μL PCR 扩增实例： 一、plasmid DNA 扩增 1．提取的质粒 DNA 扩增 以pUC19 质粒为模板扩增 100，250，500
Crystallization of Kinesin Family Motor Proteins
)Vapor diffusion15 mg/ml protein co-crystalized with 5 mM AMPPCP + 20 mM MgCl2 in reservoir buffer (RB3) composed of 30% w/v PEG4000, 100 mM Tris-HCl pH 8.5, 200 mM sodium acetateKikkawa et al., 2001Nkin 1-355Sitting Drop 19°C7.5-15 mg/ml protein in 20 mM
Single tube confirmation PCR protocol
(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution
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