- 询价
- TRC
- 进口原装
- TRC-E925195
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Ethyl (4-Nitrophenyl)acetate
- 库存：
1
- 供应商：
甄准生物
- CAS号：
5445-26-1
- 保存条件：
Refrigerator
- 规格：
5g / 50g
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文献和实验of paraoxonase-1 activity in lysate The bioactivity of paraoxonase-1 present in the cell lysate was demonstrated by its ability to hydrolyse 4 mM phenyl acetate in 20 mM Tris HCl buffer pH 8.0, containing 1 mM CaCl2. As a control 10 mM EDTA was added
tube mix: 15 µl dH2O (times number of samples + 1) 70 µl 1MTris Ph 8.0 (times number of samples + 1) 20 µl 4 mM Acetyl CoA (times number of samples + 1) and 5 µl 14 C CAM (times number of samples). 13. Add 0.5 ml Ethyl Acetate to each tube. 14
Ethyl Acetate to each tube. 14. Vortex 10" each tube & then mix by inverting for another 30". 15. Spin @ 14K RPM for 30"-1'. 16. Remove 450 µl of organic (upper) phase. 17. Speed vac for 15'. 18. Resuspend
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