- 询价
- TRC
- 进口原装
- TRC-D558020
- 2025年07月14日
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- 文献和实验
- 技术资料
- 英文名：
Doxorubicinol Hydrochloride (Mixture of diastereomers) Discontinued See: D558021
- 库存：
1
- 供应商：
甄准生物
- CAS号：
63950-05-0
- 保存条件：
-20°C Freezer, Under Inert Atmosphere
- 规格：
2mg / 20mg
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文献和实验Construction of Gene-Targeting Vectors by Recombineering
region is then retrieved from the BAC into PL611, which contains the Amp R gene. (D ) The Bsd cassette is then replaced by a reporter (i.e., the lacZ reporter cassette) by a simple restriction digestion and ligation process. The final targeting vector
(Invitrogen) at a 1:3 ratio (e.g. 1µl plasmid to 3µl lipofectamine). The plasmid and lipofectamine are mixed together, vortexed, pulse spun, and then 50µl of DMEM (without Fetal bovine serum, FBS) added. The entire mixture is again vortexed followed by a pulse
supernatant is incubated with diatomaceous earth and guanidine hydrochloride and this mixture is added into one of the twenty-four wells in the BioRad Gene Prep Manifold. The supernatant is removed by vacuum filtration over a nitrocellulose filter. The DNA
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