HK Yellow-AM (6/12混合物),HKYello

Microdeletion screening

Fig. 43. Y-chromosome microdeletion screening reactions of 12 male genomic DNA samples (yellow) using multiplex mixture B (5 loci) and of 11 DNA samples (green) using multiplex mixture A (7 loci). DNA samples 1, 2, 9, 10 and 12 (yellow

（共享）ELISA原理与实验方法

)9 Incubate 30 min at 37°C.10 Rinse 3x with 0.05% Tween 80 in 0.01 M PBS, pH 7.4.11 Rinse 3x with HPLC water.12 Add 50 µl of color development reagent(1 tablet of p-nitrophenyl reagent in 5 ml of 10% diethanolamine, pH 9.8) (mix well)13 Incubate 30 min at 37°C

Cell Cultures

.EukaryotesBy contrast to the simple broth cultures of E. coli, the nutrient requirements of even the simplest eukaryotic culture are complex. Refer to Table 12.1 for a comparison of the ingredients of Nutrient Broth and Minimum Essential Media (MEM), a typical