PSMA/FOLH1重组蛋白|Recombinant Human PSMA/FOLH1 Protein (ECD, AVI & His Tag), Biotinylated

PSMA/FOLH1重组蛋白|Recombinant Human PSMA/FOLH1 Protein (ECD, AVI & His Tag), Biotinylated（产品说明）
蛋白名称：Human PSMA/FOLH1 Protein (ECD, AVI & His Tag), BiotinylatedA DNA sequence encoding the Human FOLH1 (NP_004467.1) (Lys44-Ala750) was expressed with an N-terminal AVI tagged polyhistidine tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：CHO Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).1.Immobilized Monoclonal anti-human PSMA Antibody, human IgG4 at 2 μg/mL (100 μL/well) can bind Recombinant Human PSMA/FOLH1 Protein (ECD, AVI & His Tag), Biotinylated (Cat: 15877-H44C-B), the EC50 is 1.5-4.5 ng/mL (QC tested). 2.Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >800 pmol/min/µg.< 1.0 EU per μg protein as determined by the LAL method.GlyThe recombinant Human FOLH1 comprises 757 amino acids and has a predicted molecular mass of 84.29 kDa. It migrates as approximately 88 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 200.3 kDa verified by SEC-MALS (Routinely tested).NP_004467.1Lys44-Ala750