氘3-3'-脱氧-3'-氟胸苷 D3-3’-Deoxy-3

Immunofluorescence and Fluorescent In Situ Hybridization in Mouse Fibroblasts and Embryonic Stem Cells

  Introduction Techniques routinely used in our lab for analysing the X-inactivation process in differentiating ES cells are described. We focus particularly on chromatin changes (histone modifications, protein association

时间分辨荧光免疫分析技术

部分与游离部分分开后，利用时间分辨荧光分析仪，即可测定复合物中的稀土离子发射的荧光强度，从而确定待测抗原的量。正常情况下，免疫复合物中的稀土离子自身荧光信号很微弱，若加入一种酸性增强液，稀土离子从免疫复合物中解离出来，和增强液中的β-二酮体、三正辛基氧化膦、Triton X-100等成分形成一种微囊。后者被激发光激发后，则稀土离子可以发出长寿命的极强的荧光信号，使原来微弱的荧光信号增强将近100万倍。采用时间分辨技术测量荧光，采用了门控技术，它是使背景荧光信号降低到零以后，再测定长寿命标记物的荧光。三

Worm genomic Southern blots

out of the freezer to thaw. - Put 50 ng DNA to be labelled in a total volume of 18.9 µl H20. This is usually 1-4 µl of a typical cosmid prep. It is unnecessary to cut cosmid DNA prior to labelling. For labelling fragments from a restriction digest: I prefer