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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷冻-20℃
- 英文名:
pentadecanoyl Coenzyme A ammonium salt
- 库存:
999+
- 供应商:
上海维亦特生物科技有限公司
- CAS号:
24870-39-1
别名:pentadecanoyl Coenzyme A ammonium salt
分子式:C36H73N10O17P3S
分子量:1042.409
稳定性:1 Year
存储:冷冻-20°C
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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient
% ethanol Measure the volume of the DNA sample. Adjust the salt concentration by adding 1/10 volume of sodium acetate, pH 5.2, (final concentration of 0.3 M) or an equal volume of 5 M ammonium acetate (final concentration of 2.0-2.5 M
Plasmid DNA Isolation, Alkaline Lysis(碱裂解法分离质粒DNA)
and then 1/3 volume 7.5 M ammonium acetate. Precipitate DNA with 2 volumes of cold ethanol. Dry pellet under vacuum. Step number 7 can be omitted if needed. Pellet can also be washed 70% ethanol to remove salt prior to drying. Modification of Birnboim and Doly Additional
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