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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Biotin anti-mouse/human NGF
- 抗体英文名:
Biotin anti-mouse/human NGF
- 浓度:
0.5 mg/mL
- 应用范围:
免疫荧光,流式
- 宿主:
小鼠
- 供应商:
上海善然生物
- 库存:
4-5周
- 抗原来源:
小鼠
- 保质期:
2-3年左右
- 适应物种:
Human Reported Reactivity Mouse, Cow, Viper
- 标记物:
Biotin
- 克隆性:
单克隆
- 保存条件:
2-8度
- 形态:
液体
- 规格:
50ug
Biotin anti-mouse/human NGF Antibody
JKhNGF-2
Catalog# / Size 509801 / 50 µg
509802 / 500 µg
Clone
Regulatory Status RUO
Other Names Nerve growth factor, NGF, β-NGF
Isotype Mouse IgG1
Description Nerve growth factor (NGF) is a small secreted protein that is important for the growth,
maintenance, and survival of certain target neurons (nerve cells). NGF consists of three types
of subunits, α, β and γ, which specifically interact to form a 7S, 130,000 molecular weight
complex. This complex contains two identical 118-amino acid β-chains, which are solely
responsible for nerve growth stimulating activity of NGF. NGF is essential for the development
and phenotypic maintenance of neurons in the peripheral nervous system (PNS) and for the
functional integrity of cholinergic neurons in the central nervous system (CNS).
Product Details
Verified Reactivity Human
Reported Reactivity Mouse, Cow, Viper
Antibody Type Monoclonal
Host Species Mouse
Immunogen recombinant human NGF (c-terminus)
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with biotin under optimal
conditions.
Concentration 0.5 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
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Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
A. DAY 1 PROCEDURE• ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUMThe Goat Anti-Mouse (GAM) NGF polyclonal antibody recognizes 2.5B NGF and the Goat IgG (GIG) preimmune serum serves as a control for the antibody (Hazleton). The relative signal
in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
genomic regions in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
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