- ￥19852.38
- corning
- 美国
- 356717
- 2025年09月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Corning PureCoat Amine 96 Well Black/Clear Flat Bottom Plate, 5/Pack, 50/Case
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
50
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文献和实验1. Mouse L-cells (ATCC CCL-1.3 or CRL-2648). 2. Cell culture medium: Same as Section 2.1.2. 3. 96-well tissue culture plate (flat well). 4. Wnt3A samples (CM, fractions from Sections. 2.3 and 2.4 ). 5. PBS. 6. Lysis Buffer: 50 mM Tris-HCl, pH
. After electrophoresis, remove the buffer wells, the tape, and pry the gel plates apart. The gel should adhere to back plate. Blot the gel to a 40 cm X 20 cm sheet of 3MM Whatman paper, cover with plastic wrap, and dry on a Hoefer gel dryer for 25 minutes at 80℃ 11
at 22 mA long 4%, 0.15 mm X 70 cm X 20 cm 8-9 hours at 15 mA long 4%, 0.15 mm X 70 cm X 20 cm 20-24 hours at15 mA 10. After electrophoresis, remove the buffer wells, the tape, and pry the gel plates apart. The gel should adhere to back plate
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