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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Corning® Intestinal Differentiation Media Pack, Kit
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
1
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文献和实验Vybrant® DyeCycle Green and Orange Stains
or Vybrant® DyeCycle™ Orange stain from the freezer and allow it to equilibrate to room temperature. Prepare flow cytometry tubes each containing 1 mL of cell suspension in complete media at a concentration of 1 × 106 cells/mL. To each tube
suspension culture dish (Corning Ultra Low attachment dishes work best for FBS EBs). Change media every 2 days by sedimentation as described above. Resuspend in 7ml differentiation medium. Culture for 8 days. Time of differentiation
Vybrant® DyeCycle Violet Stain
if media is not desired, however phosphate buffers are not recommended Do not use glass containers with this stain Do not wash or fix cells after staining cells with Vybrant® DyeCycle™ Violet stain Validate flow cytometry instrument
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