CheKine™ 精氨酸（Arg）含量检测试剂盒（微量法）

CheKine™ Micro Arginine (Arg) Content Assay Kit

精准定量精氨酸代谢水平，支持多类型样本分析

产品背景与原理

精氨酸（Arginine, Arg）作为半必需氨基酸，在蛋白质合成、一氧化氮生成及血糖调节中发挥关键生理作用。本试剂盒通过特异性显色反应定量检测样本中Arg含量：在碱性介质中，Arg与α-萘酚和2,3-丁二酮反应生成红色产物，该产物在525 nm波长处具特征吸收峰，通过吸光度值精准计算Arg浓度。

核心优势

• 广谱样本适用——经验证适用于动植物组织、细菌、细胞及液体样本
• 高灵敏度检测——最低检测限达31.25 nmol/mL
• 操作流程简化——优化的试剂组分确保实验流程高效便捷

试剂盒组分

• Extraction BufferⅠ
• Extraction Buffer II
• ReagentⅠ
• ReagentⅡ
• ReagentⅢ
• Standard

关键技术参数

操作注意事项

• 推荐使用新鲜样本，如需保存请于-80℃存储（≤1个月）
• 实验前确保所有试剂及设备达到规定温度
• 避免反复冻融试剂

应用提示

适用于代谢研究、疾病机制探索及药物开发等领域中精氨酸水平检测，检测结果可关联：

• 一氧化氮合成通路活性评估
• 血糖调节机制研究
• 血管功能相关性分析

重要声明：本产品仅用于科研目的，不适用于人类或临床诊断。使用前请查阅相关专利许可限制，产品使用超出许可范围导致的侵权责任由使用者承担。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Arginine (Arg) Content Assay Kit

Accurate Quantification of Arginine Metabolic Levels with Support for Multiple Sample Types

Background and Principle

Arginine (Arg), a semi-essential amino acid, plays critical physiological roles in protein synthesis, nitric oxide production, and blood glucose regulation. This kit quantitatively measures Arg content through a specific chromogenic reaction: under alkaline conditions, Arg reacts with α-naphthol and 2,3-butanedione to form a red product with characteristic absorbance at 525 nm, enabling precise concentration calculation via absorbance values.

Key Advantages

• Broad Sample Applicability – Validated for animal/plant tissues, bacteria, cells, and liquid samples
• High Sensitivity – Minimum detection limit of 31.25 nmol/mL
• Streamlined Workflow – Optimized reagent components ensure efficient and user-friendly procedures

Kit Components

• Extraction BufferⅠ
• Extraction Buffer II
• ReagentⅠ
• ReagentⅡ
• ReagentⅢ
• Standard

Technical Parameters

Operational Notes

• Fresh samples are recommended; store at -80°C if necessary (≤1 month)
• Ensure all reagents and equipment reach specified temperatures before use
• Avoid repeated freeze-thaw cycles of reagents

Application Guidance

Suitable for arginine level detection in metabolic studies, disease mechanism exploration, and drug development. Results can correlate with:

• Nitric oxide synthesis pathway activity assessment
• Blood glucose regulation mechanism research
• Vascular function correlation analysis

Important Notice: This product is for research use only and not intended for human or clinical diagnosis. Users must review relevant patent licensing restrictions and assume liability for any infringement resulting from unauthorized use.

Note: Above information may change due to product optimization. For updates, consult Yakonin Company.