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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
冷冻-20℃
- 英文名：
Transfection Reagent I, contains DOTAP:DOPE (1:1 w/w)
- 库存：
999+
- 供应商：
上海维亦特生物科技有限公司
- CAS号：
N/A
别名：Transfection Reagent I, contains DOTAP:DOPE (1:1 w/w)
吸湿性：Yes
稳定性：1 Year
存储：冷冻-20°C
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文献和实验Transient transfection into 293T cells
by the following Ca3 (PO4 )2 transfection protocol. Up to 50% efficiency is attainable. Materials • Complete medium: DMEM(high glucose) supplemented w/ 10% FCS (either w/ or w/o heat inactivation at 55°C/30min), NEAA, Na-pyruvate, and Gln (Pen/Streptmycin
Transient transfection into 293T cel
. They (both) have the unusual property of being highly transfectable by the following Ca3 (PO4 )2 transfection protocol. Up to 50% efficiency is attainable.Materials• Complete medium: DMEM(high glucose) supplemented w/ 10% FCS (either w/ or w/o heat inactivation at 55°C/30min
Transient transfection into 293T cells
(either w/ or w/o heat inactivation at 55°C/30min), NEAA, Na-pyruvate, and Gln (Pen/Streptmycin: optional) • Transfection reagents: i) 2M CaCl2 : 2M in H2 O, filter sterile, store at 4°C ii) x2HBS: 8.0g NaCl, 0.37g KCl, 201mg (yes! mg) Na
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