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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷冻-20℃
- 英文名:
Fluorescent Transfection Reagent, contains DOTAP:DOPE (1:1, w/w) + 0.1 wt.% NBD-DOPE
- 库存:
999+
- 供应商:
上海维亦特生物科技有限公司
- CAS号:
N/A
别名:Fluorescent Transfection Reagent, contains DOTAP:DOPE (1:1, w/w) + 0.1 wt.% NBD-DOPE
吸湿性:Yes
光敏性:Yes
稳定性:1 Year
存储:冷冻-20°C
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文献和实验v468.Chapter 8 采用 b-Catenin/TCF基本转录通讯结构对b-Catenin/TCF转录进行分析
3. Lipofectamine 2000 (Invitrogen , Carlsbad, CA; cat. #11668-027) or transfection reagent of choice. 4. Optimem (Invitrogen; cat. #31985-088). 5. Plasmids (see Notes 2 and 3 ): pGL3BARL, pGL3fuBARL, pRLTK(Promega, Madison, WI; cat. #E2241), cDNA
At least 6 x 106 cells are needed to proceed with the transfection. 8. Plate 3 x 106 mES cells in 10 mL of mES cell growth medium with LIF into a gelatin-coated, 10-cm tissue culture dish. Prepare two 10-cm dishes per transfection.
a subset of genomic DNA sequences of type 5'-(NNN )3 -3', whereas the 6ZF library would recognize a subset of genomic sequences of type 5'-(NNN )6 -3'. Given the zinc-finger domains used, both libraries were more likely to recognize (RNN)x -type sequences
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![1,1’-[Iminobis(carbonimidoyliminohexane-6,1-dil)]bis[5-(4-chlorophenyl)biguadine]-d4](https://img1.dxycdn.com/p/s14/2025/0412/851/0466313177692190191.jpg!wh200)

