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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
冷冻-20℃
- 英文名：
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000] ammonium salt
- 库存：
999+
- 供应商：
上海维亦特生物科技有限公司
- CAS号：
385437-57-0
别名：1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000] ammonium salt
吸湿性：Yes
分子式：C142H280N5O56PS (average MW due to polydispersity of PEG)
分子量：3016.815
稳定性：1 Year
存储：冷冻-20°C
溶解性：Solubility in Different SolventsSoluble in ethanol, DMSO, and Chloroform:Methanol (85:15) at 5mg/mL
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文献和实验of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
expressing plasmid of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine
Crystallization of Kinesin Family Motor Proteins
temperature11 mg/ml protein 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM MgCl2, 0.2 mM NaN3, 2 mM ADP combined 1:1 with 22% methyl ether PEG 2000, 100 mM NaCl, 2% ethylene glycol, 50 mM HEPES pH 7.0Gulick et al., 1998Ncd 281-700Sitting drop 4°C20 mg/ml
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