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文献和实验ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
to get through these as quickly as possible. 1. Spin down beads @10K, 1min, RT. 2. Aspirate supernatant. 3. Wash with 1ml of the following buffers and mix by inverting 3 or 4 times. Only aspirate supernatant to 0.1ml marking so no beads are lost: o FA lysis buffer
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
L PMSF solution, 10 μL sodium orthovanadate solution and 10 μL protease inhibitor cocktail solution to 1ml of 1X RIPA buffer to prepare complete RIPA Lysis buffer Pour off media from tissue culture dish into waste container Wash
once with 20 ml Pbs transfer to 2 ml Eppendorf tube, wash again with Pbs and freeze cells or proceed on resuspend in 200 - 400 µl CHIP lysis buffer , add an equal volume of glass beads shake for 30 min at 4 °C on vortexer (maximum
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