- ￥4603
- BioLegend
- 201516
- 美国
- 2025年07月15日
- 免疫荧光，流式
- 小鼠
- 大鼠
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- 抗体名：
PE/Cyanine7 anti-rat CD4
- 抗体英文名：
PE/Cyanine7 anti-rat CD4
- 浓度：
0.2 mg/ml
- 应用范围：
免疫荧光，流式
- 宿主：
小鼠
- 供应商：
上海善然生物
- 库存：
现货
- 抗原来源：
小鼠
- 保质期：
2-3年左右
- 适应物种：
大鼠
- 标记物：
PE/Cyanine7
- 克隆性：
单克隆
- 保存条件：
2-8度
- 形态：
液体
- 规格：
100 μg
PE/Cyanine7 anti-rat CD4 Antibody
W3/25
Catalog# / Size 201515 / 25 µg
201516 / 100 µg
Clone
Regulatory Status RUO
Other Names T4, L3T4, W3/25
Isotype Mouse IgG1, κ
Description CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin
superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell
subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II
molecule and is involved in T cell activation.
Product Details
Verified Reactivity Rat
Antibody Type Monoclonal
Host Species Mouse
Immunogen Rat thymocyte membrane glycoproteins
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under
optimal conditions.
Concentration 0.2 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
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Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
Most T lymphocytes of the immune system differentiate within the thymus along the CD4/CD8 developmental pathway by a highly ordered process termed thymic selection ( 1 , 2 ). The maturation status of thymocytes is commonly assessed
CD3/ CD4 /CD8 T cell subset counts
Description Procedure for CD3/ CD4 /CD8 T cell subset counts using CD3-FITC / CD4-PE / CD8-PECy5 antibody Procedure 1. One 5 ml round bottom tube (Falcon) is taken per blood sample to be analyzed. The tube is labeled, which includes
69、CD38 等活化指标，及 IFN-γ、IL-4、IL17A 等细胞因子，则需要设置同型对照，实现阴阳性细胞群的良好区分。 CD4 CD25 （2）在某些特殊情况下，如单核细胞被 PE/Cyanine 7、APC/Cyanine 7 等串联染料标记的抗体染色时，使用同型对照抗体是十分有必要的。单核细胞一般倾向于与 Cyanine7 等染料非特异性结合，使用同型对照抗体有助于获得理想结果。 （3）不清楚抗原表达情况的时候，一定要使用同型对照来界定阴性细胞群。 （4）胞内染色时
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