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文献和实验E.Z.N.A. Plant RNA Midi Protocol II (for difficult samples)
, including any precipitates that may form to an HiBind® RNA Midi column assembled in a clean 15 ml collecting tube (supplied). Centrifuge at 4,000 x g for 5 minutes at room temperature. Discard the flow-through liquid and place the column
E.Z.N.A.® Total RNA Midi Kit Protocol for Eukaryotic Cells and Tissues
-milling or rotor-stator homogenizer, samples are then applied to the HiBind® Midi spin columns to which total RNA binds, after few quick washing step, cellular debris and other contaminants are effectively washed away. High quality RNA is finally eluted
Materials Qiagen RNEasy Midi Kit Dounce homogenizers (for tissues) Syringes and needles (for cultured cells) 70% EtOH Sterile 15 mL conical tubes Procedure 1) Before using the reagents
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