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- B21841401
- 2025年12月18日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
We recommend that you refer to the Storage method in the COA
- 保质期：
见产品包装
- 英文名：
Recombinant Human CA9(38-414)/ His Protein
- 库存：
充分
- 供应商：
百英生物
-
Alternative Name
Carbonic Anhydrase IX
-
Target
CA9
-
Source
Human CA9 was expressed from HEK293 with poly His at the C-terminal
-
Sequence
Gln38-Asp414
-
Protein structure:
Q16790His
-
Molecular Characterization:
The protein has a calculated MW of 42kDa.The reducing (R) heterodimer protein migrates as 50-70 kDa due to glycosylation
-
Purity:
SDS-PAGE>95%，SEC>90%
-
Reconstitution:
sterile H2O
-
Endotoxin:
<1EU/mg
-
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
-
Alternative Name
Carbonic Anhydrase IX
-
Specificity
-
Host Cell:
-
Accession:
Q16790
-
Delivery:
-
Positive Rate:
-
Mycoplasma Testing
-
Storage:
We recommend that you refer to the Storage method in the COA
-
Growth/Thaw Medium:
-
Vector:
Determined by SDS-PAGE, the purity of the protein is greater than 95%.
Determined by SEC-HPLC, the purity of the protein is greater than 95%.
When Recombinant Human CA9（38-414）/His Protein is immobilized at 2µg/mL (100 µL/well), the concentration of Anti-Human CA9 (Girentuximab) that produces 50% of the optimal binding response is approximately 9 ng/ml.
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文献和实验High Throughput Production of Recombinant Human Proteins for Crystallography
, to be applied based on specific knowledge of protein families or when the core protocol is unsatisfactory. This scheme has been applied to more than 550 human proteins (>10,000 constructs) and has resulted in the deposition of 112 unique structures
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
, H. 2009. Profiling the human protein‐DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling. Cell 139:610‐622. Hudson, J.R., Jr., Dawson, E.P
Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
Bait Protein Multivalent Complexes on Protein A Microbeads Human IgG (Jackson ImmunoResearch, cat. no. 009‐000‐003
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