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- 140417
- 2025年07月14日
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- 技术资料
- 抗体名：
PerCP/Cyanine5.5 anti-mouse CD8b.2
- 抗体英文名：
PerCP/Cyanine5.5 anti-mouse CD8b.2
- 标记物：
PerCP/Cyanine5.5
- 规格：
25ug
PerCP/Cyanine5.5 anti-mouse CD8b.2 Antibody
53-5.8
Catalog# / Size 140417 / 25 µg
140418 / 100 µg
Clone
Regulatory Status RUO
Other Names Lyt-3.2, Ly-3.2
Isotype Rat IgG1, κ
Description CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig
superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class Irestricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in
T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.
Product Details
Isotype Control PerCP/Cyanine5.5 Rat IgG1, κ Isotype Ctrl
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Mouse thymus or spleen
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5
under optimal conditions.
Concentration 0.2 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
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monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
Purification Of Anti Peptide Antibody
a parastaltic pump and recirculate the serum ON or just batch bind the serum ON. Wash with 5 col vols TBS. Wash with 10 col vols 0.5 M NaCl, 20mM TrisCl pH 7.4, 0.2 Triton- X-100. Wash 5 col vols TBS Elute with 0.15 M NaCl, 0.2 M Glycine
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
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