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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
PerCP/Cyanine5.5 anti-mouse CD4
- 抗体英文名:
PerCP/Cyanine5.5 anti-mouse CD4
- 浓度:
0.2 mg/ml
- 应用范围:
免疫荧光,流式
- 宿主:
大鼠
- 供应商:
上海善然生物
- 库存:
现货
- 抗原来源:
大鼠
- 保质期:
2-3年左右
- 适应物种:
小鼠
- 标记物:
PerCP/Cyanine5.5
- 克隆性:
单克隆
- 保存条件:
2-8度
- 形态:
液体
- 规格:
100 μg
PerCP/Cyanine5.5 anti-mouse CD4 Antibody
GK1.5
Catalog# / Size 100433 / 25 µg
100434 / 100 µg
Clone
Regulatory Status RUO
Other Names L3T4, T4
Isotype Rat IgG2b, κ
Description CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily,
primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and
dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic
differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.
Product Details
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Mouse CTL clone V4
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5
under optimal conditions.
Concentration 0.2 mg/ml
Storage & Handling The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
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Th17 Polarization of Mouse CD4 Cells
in complete medium and use your favorite method to isolate CD4 cells. Check out Biocompare.com to find useful kits. 2. Th17 Polarization of CD4 cells 1) Coat 60×15 mm of plastic petri dishes with anti-mouse CD3ε, clone 145-2C11(2&mu
Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
Most T lymphocytes of the immune system differentiate within the thymus along the CD4/CD8 developmental pathway by a highly ordered process termed thymic selection ( 1 , 2 ). The maturation status of thymocytes is commonly assessed
and H2-A and E in mice) for CD4+ T cells. However, a significant proportion of T cells recognize peptide antigens bound to nonclassical MHC class Ib molecules such as the human HLA-E (mouse analog Qa1) (4 ) and mouse H2-M3 (5 ). In addition, some T
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