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文献和实验MNAzyme qPCR: A Superior Tool for Multiplex qPCR
with a generic readout. A series of universal probes have been designed that perform with high reliability, yielding consistent and reproducible results for any target, making the development of multiplex qPCR assays faster, cheaper, and simpler. This chapter
.Optional: Confirm successful protein transfer by Ponceau Red staining before proceeding to the next step.封闭,抗体孵育和洗膜1. Blocking: Incubate the blot in 3% non-fat milk / PBST or Trident Universal Protein Blocking Reagent for 30-60 minutes at RT.2. Primary
-32P]dNTP或DIG-11-dUTP,所增殖的DNA即被32P或DIG所标定,所以PCR是制备核酸探针非常便捷好用的方法。仪器用具:微量离心机;PCR 反应器药品试剂:模版DNA: pBlueGus 质体DNA (~50 pg)核酸引子对: T3 promoter primer 与T7 promoter primer (各2 μM)矿物油 (是否需要视PCR反应器机型而定)PCR DIG probe synthesis kit (Roche), 内含:Taq DNA polymerase
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